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Phospholipase A2 from Vipera ammodytes meridionalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Spacegroup name	P 21 21 21
Unit cell lengths	46.568, 82.670, 119.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.530 - 3.300
R-factor	0.234
Rwork	0.234
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.530
High resolution limit [Å]	3.300
Number of reflections	6922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG-8000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K