1RG0
Monoclinic crystal form of the truncated K122-4 pilin from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.301, 80.720, 39.127 |
Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
Resolution | 40.490 - 1.800 |
R-factor | 0.23661 |
Rwork | 0.236 |
R-free | 0.25428 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qve |
RMSD bond length | 0.006 |
RMSD bond angle | 2.169 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.490 | 1.630 |
High resolution limit [Å] | 1.510 | 1.510 |
Number of reflections | 25475 | |
<I/σ(I)> | 9.3 | 0.6 |
Completeness [%] | 78.5 | 68 |
Redundancy | 2.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | PEG 4000, Potassium Phosphate Monobasic, Sodium Cacodylate, Tris HCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |