1RG0
Monoclinic crystal form of the truncated K122-4 pilin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.301, 80.720, 39.127 |
| Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
| Resolution | 40.490 - 1.800 |
| R-factor | 0.23661 |
| Rwork | 0.236 |
| R-free | 0.25428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qve |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.169 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.490 | 1.630 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 25475 | |
| <I/σ(I)> | 9.3 | 0.6 |
| Completeness [%] | 78.5 | 68 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | PEG 4000, Potassium Phosphate Monobasic, Sodium Cacodylate, Tris HCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
