1RFZ
Structure of Protein of Unknown Function from Bacillus stearothermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-06 |
Detector | SBC-2 |
Wavelength(s) | 0.97956, 0.97943, 0.9647 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.824, 80.824, 236.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.770 - 2.800 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.27700 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.107 | 0.792 |
Number of reflections | 22606 | |
Completeness [%] | 100 | |
Redundancy | 15.5 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | ammonium sulfate, BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |