1RFS
RIESKE SOLUBLE FRAGMENT FROM SPINACH
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-14 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 29.050, 31.870, 35.790 |
Unit cell angles | 95.60, 106.10, 117.30 |
Refinement procedure
Resolution | 20.000 - 1.830 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.22000 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.920 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.048 | 0.108 |
Total number of observations | 28882 * | |
Number of reflections | 8758 | |
<I/σ(I)> | 10.1 | 6.5 |
Completeness [%] | 89.7 | 57.3 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 100 (mM) | |
2 | 1 | reservoir | ammonium acetate | 100-200 (mg/ml) | |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | drop | sodium acetate | 50 (mM) | |
5 | 1 | drop | ammonium acetate | 50-100 (mg/ml) | |
6 | 1 | drop | PEG4000 | 15 (%) | |
7 | 1 | drop | protein | 15 (mg/ml) | |
8 | 1 | drop | MOPS | 10 (mM) |