1RFJ
Crystal Structure of Potato Calmodulin PCM6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-29 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.440, 68.980, 109.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.950 - 2.000 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.129 |
Number of reflections | 12583 | |
<I/σ(I)> | 23.5 | 6.1 |
Completeness [%] | 95.1 | 88.9 |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 277 | MPD, CaCl2, NaAc-HAc, pH 3.90, VAPOR DIFFUSION, HANGING DROP, temperature 277K |