1RFG
Crystal Structure of Human Purine Nucleoside Phosphorylase Complexed with Guanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 104 |
Detector technology | CCD |
Collection date | 2003-06-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.4310 |
Spacegroup name | H 3 2 |
Unit cell lengths | 139.380, 139.380, 160.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.900 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pwy |
RMSD bond length | 0.018 |
RMSD bond angle | 2.003 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.224 | 3.010 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.043 | 0.214 |
Number of reflections | 14265 | |
Completeness [%] | 95.8 | 97.6 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | AMMONIUM SULFATE, pH 5.30, VAPOR DIFFUSION, HANGING DROP, temperature 298K |