1RFE
Crystal structure of conserved hypothetical protein Rv2991 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 49.309, 49.309, 132.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.000 |
| R-factor | 0.20572 |
| Rwork | 0.202 |
| R-free | 0.28106 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.689 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.390 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.177 | 0.790 |
| Number of reflections | 127155 | |
| <I/σ(I)> | 11.68 | 1.23 |
| Completeness [%] | 98.8 | 76.7 |
| Redundancy | 10.87 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 25% PEG 4000 , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
