1RFB

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE INTERFERON-GAMMA AT 3.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	42.800, 79.900, 85.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 3.000
R-factor	0.192
Rwork	0.192
RMSD bond length	0.017
RMSD bond angle	0.052 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.660 *
Total number of observations	19800 *
Number of reflections	7891 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *	17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11.0 (mg/ml)
2	1	drop	ammonium acetate	100 (mM)
3	1	drop	ammonium sulfate	30 (%sat)
4	1	reservoir	ammonium sulphate	50 (%sat)