1RF3
Structurally Distinct Recognition Motifs in Lymphotoxin-B Receptor and CD40 for TRAF-mediated Signaling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 83.700, 83.700, 79.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 3.500 |
Rwork | 0.266 |
R-free | 0.31500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1l0a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.520 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 3177 | |
<I/σ(I)> | 8 | 8 |
Completeness [%] | 95.4 | 95 |
Redundancy | 4.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 15% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |