1RF0
Crystal Structure of Fragment D of gammaE132A Fibrinogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98389 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.408, 94.948, 227.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 * - 2.800* |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28600 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1lt9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.240 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 * | 0.408 * |
| Total number of observations | 186713 * | |
| Number of reflections | 46581 | |
| <I/σ(I)> | 13 | 3.4 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 * | 4 * | 14-15% PEG 3350, 70 mM calcium chloride, 2 mM sodium azide, 50 mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | Tris | 50 (mM) | pH8.5 |
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | reservoir | 70 (mM) | ||
| 6 | 1 | reservoir | PEG3350 | 14-15 (%) |
