1REU

Structure of the bone morphogenetic protein 2 mutant L51P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-04-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	94.292, 94.292, 102.882
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 2.650
R-factor	0.2151
Rwork	0.215
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bmp
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	2.820
High resolution limit [Å]	2.650	2.650
Rmerge	0.060
Number of reflections	5624
<I/σ(I)>		2.4
Completeness [%]	93.5	72.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	lithium sulfate, tert-butanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K