1REP
CRYSTAL STRUCTURE OF REPLICATION INITIATOR PROTEIN REPE54 OF MINI-F PLASMID COMPLEXED WITH AN ITERON DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.435, 81.853, 73.891 |
| Unit cell angles | 90.00, 121.53, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.27400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.035 | 0.298 |
| Total number of observations | 57565 * | |
| Number of reflections | 15208 | |
| <I/σ(I)> | 34.7 | 4.8 |
| Completeness [%] | 88.7 | 65 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | Komori, H., (1999) J.Biochem.(Tokyo), 125, 24. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | TRIS-HCL | ||
| 4 | 1 | 2 | PEG 400 | ||
| 5 | 1 | 2 | MGCL2 | ||
| 6 | 1 | 2 | TRIS-HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG400 | 12 (%) | |
| 4 | 1 | reservoir | 200 (mM) |
