1REF
ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 2,3-EPOXYPROPYL-BETA-D-XYLOSIDE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1995-10-02 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.340, 60.880, 37.890 |
Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.22900 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 * |
Data reduction software | R-AXIS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.086 | |
Total number of observations | 82450 * | |
Number of reflections | 30167 | |
Completeness [%] | 85.5 | 60.3 * |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.2 * | Torronen, A., (1993) J. Mol. Biol., 233, 313. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium acetate | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 25 (%) | |
4 | 1 | reservoir | ammonium sulfate | 25 (%) |