1REE

ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 3,4-EPOXYBUTYL-BETA-D-XYLOSIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-07-18
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	81.840, 60.810, 38.090
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.181
Rwork	0.181
R-free	0.22300
RMSD bond length	0.006
RMSD bond angle	1.400 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.091
Total number of observations	84926 *
Number of reflections	35521
Completeness [%]	85.1	57.5 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2 *		Torronen, A., (1993) J. Mol. Biol., 233, 313. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	drop	ammonium sulfate	25 (%)
4	1	reservoir	ammonium sulfate	25 (%)