1RE2

HUMAN LYSOZYME LABELLED WITH TWO 2',3'-EPOXYPROPYL BETA-GLYCOSIDE OF N-ACETYLLACTOSAMINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	56.450, 62.300, 32.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.171 *
Rwork	0.171
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1REZ WITH SOLVENT MOLECULESOMITTED
RMSD bond length	0.011
RMSD bond angle	24.900 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	62.300
High resolution limit [Å]	2.250
Rmerge	0.111
Total number of observations	23021 *
Number of reflections	5742
<I/σ(I)>	3.7
Completeness [%]	96.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium nitrate	3 (M)
2	1	1	sodium acetate	25 (mM)
3	1	1	protein	20 (mg/ml)
4	1	2	ammonium nitrate	5 (M)