1RDZ
T-STATE STRUCTURE OF THE ARG 243 TO ALA MUTANT OF PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 296 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.060, 166.780, 79.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.050 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.390 * |
| Data reduction software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.000 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.079 |
| Number of reflections | 47244 |
| Completeness [%] | 88.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 10 | 1 | reservoir | EDTA | 0.5 (mM) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | Tris | 50 (mM) | |
| 4 | 1 | drop | PEG3500 | 15 (%) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | EDTA | 0.5 (mM) | |
| 7 | 1 | reservoir | Tris | 50 (mM) | |
| 8 | 1 | reservoir | PEG3500 | 15 (%) | |
| 9 | 1 | reservoir | 1 (mM) |
