1RDH

CRYSTALLOGRAPHIC ANALYSES OF AN ACTIVE HIV-1 RIBONUCLEASE H DOMAIN SHOW STRUCTURAL FEATURES THAT DISTINGUISH IT FROM THE INACTIVE FORM

Experimental procedure

Spacegroup name	P 31
Unit cell lengths	52.030, 52.030, 113.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.215
RMSD bond length	0.026
RMSD bond angle	0.052
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.072 *
Total number of observations	19985 *
Number of reflections	8145 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.5-10 (mg/ml)
2	1	reservoir	PEG6000	20-23 (%(w/v))
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	sodium tartrate	0.15 (M)