1RD7
DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-04-04 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.233, 65.658, 116.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| Rwork | 0.163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra2 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.900 * |
| Data reduction software | UCSD |
| Data scaling software | UCSD |
| Phasing software | MERLOT |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 * | |
| Total number of observations | 53453 * | |
| Number of reflections | 12108 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 4.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ecDHFR-FOX | ||
| 2 | 1 | drop | imidazole | 10 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | monosodium glutamate | 200 (mM) | |
| 5 | 1 | reservoir | Tris | 100 (mM) |
