1RD5
Crystal structure of Tryptophan synthase alpha chain homolog BX1: a member of the chemical plant defense system
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.934 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 91.094, 159.815, 162.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.020 |
| R-factor | 0.24804 |
| Rwork | 0.245 |
| R-free | 0.29867 |
| Structure solution method | SAD |
| RMSD bond length | 0.046 |
| RMSD bond angle | 3.021 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 37043 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 92.7 | 52.7 |
| Redundancy | 5.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | Malonate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
