1RCY
RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1993-10-20 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.510, 60.670, 38.140 |
Unit cell angles | 90.00, 108.42, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.25200 |
Structure solution method | MULTIWAVELENGTH ANOMALOUS DISPERSION |
RMSD bond length | 0.006 |
RMSD bond angle | 27.559 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.050 | |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.020 | 0.038 |
Total number of observations | 14468 * | |
Number of reflections | 9167 | |
<I/σ(I)> | 23.5 | 10.1 |
Completeness [%] | 82.0 | 63 |
Redundancy | 1.5 | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | 4 * | Djebli, A., (1992) J.Mol.Biol., 227, 581. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.5 (mg/ml) | |
2 | 1 | drop | sodium citrate | 50 (mM) | |
3 | 1 | drop | lithium chloride | 100 (mM) | |
4 | 1 | drop | PEG8000 | 12.5 (%(w/v)) | |
5 | 1 | reservoir | sodium citrate | 100 (mM) | |
6 | 1 | reservoir | lithium chloride | 200 (mM) | |
7 | 1 | reservoir | PEG8000 | 25 (%) |