1RCP

CYTOCHROME C'

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1994-12-22
Detector	FUJI
Spacegroup name	I 1 2 1
Unit cell lengths	47.185, 70.129, 88.712
Unit cell angles	90.00, 86.82, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.163
Rwork	0.163
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	19.810 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.057
Total number of observations	61516 *
Number of reflections	15826
<I/σ(I)>	20.09
Completeness [%]	79.3
Redundancy	3.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium citrate	1.5 (M)