1RCP
CYTOCHROME C'
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1994-12-22 |
Detector | FUJI |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 47.185, 70.129, 88.712 |
Unit cell angles | 90.00, 86.82, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.163 |
Rwork | 0.163 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 19.810 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.057 |
Total number of observations | 61516 * |
Number of reflections | 15826 |
<I/σ(I)> | 20.09 |
Completeness [%] | 79.3 |
Redundancy | 3.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 1.5 (M) |