1RCP
CYTOCHROME C'
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-22 |
| Detector | FUJI |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 47.185, 70.129, 88.712 |
| Unit cell angles | 90.00, 86.82, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 19.810 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.057 |
| Total number of observations | 61516 * |
| Number of reflections | 15826 |
| <I/σ(I)> | 20.09 |
| Completeness [%] | 79.3 |
| Redundancy | 3.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 1.5 (M) |
