1RC8
T4 Polynucleotide Kinase bound to 5'-GTCAC-3' ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.170, 92.061, 123.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.190 - 2.750 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ltq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.051 | 0.284 |
| Total number of observations | 92826 * | |
| Number of reflections | 11117 | |
| <I/σ(I)> | 15.7 | 5 |
| Completeness [%] | 89.1 | 91.6 |
| Redundancy | 5-6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | Galburt, E.A., (2002) Structure, 10, 1249. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | TRIS | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | EDTA | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | MES | ||
| 9 | 1 | 2 | TRIS |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | ATP | 0.1 (mM) | |
| 6 | 1 | drop | protein | 1 (mg/ml) | |
| 7 | 1 | reservoir | PEG4000 | 5-10 (%) | |
| 8 | 1 | reservoir | 0-5 (mM) | pH6.5 |
