1RBO
SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Collection date | 1989-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.200, 157.200, 201.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.300 |
| R-factor | 0.154 |
| Rwork | 0.154 |
| R-free | 0.17100 |
| Structure solution method | ISOMORPHOUS WITH 8RUC |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 | |
| Total number of observations | 488333 * | |
| Number of reflections | 108532 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 90.7 | 100 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | 1.7 M (NH4)2SO4, 50 MM PHOSPHATE, PH 7.4 3 MM MGCL2, 25 MM NAHCO3, 10 MM 4CABP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | spinach Rubisco | 100 (mg/ml) | |
| 2 | 1 | drop | 3 (mM) | ||
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | 4-CABP | 10 (mM) | |
| 5 | 1 | drop | phosphate | 50 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 7 | 1 | reservoir | phosphate | 50 (mM) |
