1RBC
CRYSTALLOGRAPHIC STRUCTURES OF RIBONUCLEASE S VARIANTS WITH NONPOLAR SUBSTITUTION AT POSITION 13: PACKING AND CAVITIES
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 44.510, 44.510, 97.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.820 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.060 * |
| Completeness [%] | 98.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.75 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 3.0 (M) | ||
| 2 | 1 | 1 | acetate | 0.5 (M) | |
| 3 | 1 | 1 | 3'CMP | 2 (mM) | |
| 4 | 1 | 1 | protein | 8-12 (mg/ml) | |
| 5 | 1 | 1 | 30-35 (%sat) |
