1RBA
SUBSTITUTION OF ASP193 TO ASN AT THE ACTIVE SITE OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE RESULTS IN CONFORMATIONAL CHANGES
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.500, 69.300, 103.100 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 6.500 * - 2.600 |
| R-factor | 0.207 |
| RMSD bond length | 0.019 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.093 * |
| Total number of observations | 57727 * |
| Number of reflections | 23071 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 4,20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 0.05 (M) | |
| 2 | 1 | 1 | 10 (mM) | ||
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | EDTA | 0.1 (mM) |
