1RB5

ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TRIGONAL FORM

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	90
Detector technology	CCD
Collection date	1997-12-05
Detector	ADSC
Wavelength(s)	1.0688, 0.9800, 0.9795, 0.9322
Spacegroup name	P 31 2 1
Unit cell lengths	89.943, 89.943, 46.481
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 1.900
R-factor	0.205
Rwork	0.202
R-free	0.25700
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	2.000
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	REFMAC (26/11/99)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	20106
Completeness [%]	99.3	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.3	298	100mM bis-tris, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	bis-tris	100 (mM)	pH7.3