1RB4

ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	90
Detector technology	CCD
Collection date	1998-06-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0688, 0.9800, 0.9797, 0.9795, 0.9322
Spacegroup name	P 43 21 2
Unit cell lengths	64.947, 64.947, 78.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.900
R-factor	0.204
Rwork	0.202
R-free	0.30700
Structure solution method	MAD
RMSD bond length	0.009 *
RMSD bond angle	0.900 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	ELVES
Refinement software	REFMAC (26/11/99)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.081 *	0.460 *
Number of reflections	15956
Completeness [%]	97.0 *	82.8 *
Redundancy	17.7 *	12.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	298	25 mM phosphate pH 7.3 400 mM NaCl 15% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphaye	25 (mM)	pH7.0
3	1	reservoir	PEG1450	12 (%(w/v))
4	1	reservoir		400 (mM)