1RAL

THREE-DIMENSIONAL STRUCTURE OF RAT LIVER 3ALPHA-HYDROXYSTEROID(SLASH)DIHYDRODIOL DEHYDROGENASE: A MEMBER OF THE ALDO-KETO REDUCTASE SUPERFAMILY

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	51.300, 89.500, 143.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.22
Rwork	0.220
RMSD bond length	0.019
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.098 *
Total number of observations	30872 *
Number of reflections	6122 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.8 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	25 (%(w/v))
2	1	reservoir	sodium citrate	100 (mM)
3	1	reservoir	ammonium sulfate	120 (mM)