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1RA9

DIHYDROFOLATE REDUCTASE COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM)

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]298
Detector technologyAREA DETECTOR
Collection date1994-12-27
DetectorXUONG-HAMLIN MULTIWIRE
Spacegroup nameC 1 2 1
Unit cell lengths74.860, 59.566, 38.914
Unit cell angles90.00, 106.49, 90.00
Refinement procedure
Resolution20.000 - 1.500

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Rwork0.169
Structure solution methodDIFFERENCE FOURIER
Starting model (for MR)1ra3
RMSD bond length0.021
RMSD bond angle3.000
Data reduction softwareUCSD
Data scaling softwareUCSD
Phasing softwareTNT
Refinement softwareTNT
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]100.0001.610
High resolution limit [Å]1.500

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1.550
Rmerge0.048

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Total number of observations75470

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Number of reflections22697
<I/σ(I)>12.82
Completeness [%]95.081.4
Redundancy3.31.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

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7macroseeding

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropecDHFR-NADP+
21dropimidazole20 (mM)
31reservoirPEG600030 (%(w/v))
41reservoir11 (mM)
51reservoir18 (mM)

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