1RA8
DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 2-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1993-01-27 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.270, 59.860, 38.920 |
Unit cell angles | 90.00, 107.38, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.151 * |
Rwork | 0.151 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1ra2 |
RMSD bond length | 0.022 |
RMSD bond angle | 3.100 |
Data reduction software | UCSD |
Data scaling software | UCSD |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.030 |
High resolution limit [Å] | 1.800 | 1.840 |
Rmerge | 0.038 * | 0.149 * |
Total number of observations | 32887 * | |
Number of reflections | 10657 | |
<I/σ(I)> | 14 | 2.7 |
Completeness [%] | 72.0 | 58 |
Redundancy | 3.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | macro seeding * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | imidazole | 20 (mM) | |
2 | 1 | reservoir | PEG6000 | 16 (%(w/v)) | |
3 | 1 | reservoir | 300 (mM) | ||
4 | 1 | reservoir | imidazole | 20 (mM) |