1R9L

structure analysis of ProX in complex with glycine betaine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-11
Detector	MARRESEARCH
Wavelength(s)	0.8439
Spacegroup name	P 21 21 21
Unit cell lengths	47.430, 53.900, 115.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.260 - 1.590
Rwork	0.150
R-free	0.18600
Structure solution method	MIR
RMSD bond length	0.013 *
RMSD bond angle	1.385 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.670
High resolution limit [Å]	1.570	1.570
Rmerge	0.026 *	0.047 *
Total number of observations	142884 *
Number of reflections	40866
Completeness [%]	96.6	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.3 *	18 *	PEG 4000, PIPES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.3
3	1	reservoir	PEG4000	26-28 (%(w/v))
4	1	reservoir	PIPES	50 (mM)	pH6.2-6.4