1R9L
structure analysis of ProX in complex with glycine betaine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8439 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.430, 53.900, 115.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.260 - 1.590 |
| Rwork | 0.150 |
| R-free | 0.18600 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.385 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.670 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.026 * | 0.047 * |
| Total number of observations | 142884 * | |
| Number of reflections | 40866 | |
| Completeness [%] | 96.6 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.3 * | 18 * | PEG 4000, PIPES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH8.3 |
| 3 | 1 | reservoir | PEG4000 | 26-28 (%(w/v)) | |
| 4 | 1 | reservoir | PIPES | 50 (mM) | pH6.2-6.4 |
