1R9F

Crystal structure of p19 complexed with 19-bp small interfering RNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-18
Detector	MARRESEARCH
Wavelength(s)	0.9791,0.9789,0.9562
Spacegroup name	H 3 2
Unit cell lengths	91.249, 91.249, 148.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 * - 1.850
R-factor	0.212
Rwork	0.212
R-free	0.23600 *
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.060	0.725
Total number of observations	194486 *
Number of reflections	20413 *	1872 *
<I/σ(I)>	44.8	2
Completeness [%]	99.1	93.2
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *	20 *	ammonium sulfate, potassium chloride, HEPES-NaOH, dithiothreitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulfate
2	1	1	potassium chloride
3	1	1	HEPES-NaOH
4	1	1	dithiothreitol
5	1	1	H2O
6	1	2	ammonium sulfate
7	1	2	potassium chloride
8	1	2	H2O

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	125000 (nM)
2	1	drop		0.1 (M)
3	1	drop	HEPES-KOH	5 (mM)
4	1	drop		10 (mM)	pH7.6
5	1	reservoir	ammonium sulfate	1.2-1.6 (M)
6	1	reservoir	HEPES-NaOH	0.1 (M)	pH7.5