1R9D
Glycerol bound form of the B12-independent glycerol dehydratase from Clostridium butyricum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.092, 213.420, 199.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.910 - 1.800 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 199038 | |
| <I/σ(I)> | 26.7 | 3.3 |
| Completeness [%] | 99.5 | 92.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | capillary batch | 6.5 | 270 | PEG 3350, tri-ammonium citrate, pH 6.5, capillary batch, temperature 270K |
