1R95

Crystal Structure of IscA (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-01-31
Detector	MARRESEARCH
Spacegroup name	P 62
Unit cell lengths	55.527, 55.527, 159.037
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.000 * - 2.600 *
R-factor	0.234
Rwork	0.232
R-free	0.25600
Structure solution method	SIR
RMSD bond length	0.010
RMSD bond angle	1.400 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.066 *	0.479 *
Total number of observations	77724 *
Number of reflections	8034
<I/σ(I)>	58	5.5
Completeness [%]	99.8	100
Redundancy	9.7	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	298	dibasic ammonium phosphate, imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	drop	ferrous ammonium sulfate	1.1 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	reservoir	dibasic ammonium phosphate	0.8-1.0 (M)
5	1	reservoir	imidazole	0.1 (M)	pH8.0