1R8W
Native structure of the B12-independent glycerol dehydratase from clostridium butyricum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.434, 212.912, 199.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.160 - 2.500 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.20300 |
| Structure solution method | Single Hg Derivative |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.160 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | |
| Number of reflections | 75084 | |
| <I/σ(I)> | 29.7 | |
| Completeness [%] | 98.4 | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Capillary Batch | 6.5 | 277 | PEG 3350, tri-ammonium citrate, pH 6.5, Capillary Batch, temperature 270K |
