1R8L
The structure of endo-beta-1,4-galactanase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.910, 81.190, 104.880 |
| Unit cell angles | 90.00, 98.53, 90.00 |
Refinement procedure
| Resolution | 19.670 - 2.600 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDN ENTRY 1FHL |
| RMSD bond angle | 0.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.670 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.101 | 0.410 |
| Number of reflections | 25224 | |
| <I/σ(I)> | 8.61 | 2 |
| Completeness [%] | 96.5 | 95.5 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 1500, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
