1R8G
Structure and function of YbdK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.470, 100.320, 71.370 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| Rwork | 0.189 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.470 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.860 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.061 | 0.103 |
| Number of reflections | 62133 | |
| <I/σ(I)> | 15.34 | 6.71 |
| Completeness [%] | 87.8 | 49.4 |
| Redundancy | 3.8 | 0.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2M NaCl, 20% w/v PEG 3350, 20mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
