1R8E

Crystal Structure of BmrR Bound to DNA at 2.4A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9537
Spacegroup name	P 43 2 2
Unit cell lengths	107.280, 107.280, 145.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.390 - 2.400
R-factor	0.227
Rwork	0.227
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1exj
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.390	2.550
High resolution limit [Å]	2.400	2.400
Number of reflections	33613
Completeness [%]	99.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	273	Imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Imidazole
2	1	1	H2O
3	1	2	Imidazole
4	1	2	H2O