1R8B

Crystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its Nucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	86.402, 79.371, 78.135
Unit cell angles	90.00, 97.33, 90.00

Refinement procedure

Resolution	38.630 - 2.000
R-factor	0.18315
Rwork	0.181
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.447
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.068 *
Number of reflections	35218
Completeness [%]	99.9 *	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.4	293	Calcium Chloride, Ethanol, Tris, magnesium chloride, sodium chloride, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	6 (mg/ml)
2	1	2		200 (mM)