1R7S
PUTIDAREDOXIN (Fe2S2 ferredoxin), C73G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.6 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.076, 78.218, 55.739 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.910 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.083 | 0.266 |
| Number of reflections | 45116 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.5M Li2SO4, 0.1M Hepes, 2nM n-octanoyl sucrose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
