1R7O

Crystal Structure of apo-mannanase 26A from Psudomonas cellulosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-01
Detector	MAR scanner 345 mm plate
Spacegroup name	P 41
Unit cell lengths	93.240, 93.240, 54.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.290 - 1.850
R-factor	0.14611
Rwork	0.145
R-free	0.17004
Starting model (for MR)	1j9y
RMSD bond length	0.009
RMSD bond angle	1.346
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.290	1.898
High resolution limit [Å]	1.850	1.850
Rmerge	0.033	0.077
Number of reflections	40381
<I/σ(I)>	29.9	10.2
Completeness [%]	99.8	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1