1R7J

Crystal structure of the DNA-binding protein Sso10a from Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	57.940, 72.300, 30.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.860 - 1.470
R-factor	0.232
Rwork	0.232
R-free	0.24400
Structure solution method	Sulfur-SAS on Cr X-ray source
RMSD bond length	0.004
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.860	1.520
High resolution limit [Å]	1.470	1.470
Rmerge	0.042	0.264
Number of reflections	21672
<I/σ(I)>	18.4	4.6
Completeness [%]	96.5	69.2
Redundancy	12.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	PEG 3350, potassium fluoride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K