1R7I
HMG-CoA Reductase from P. mevalonii, native structure at 2.2 angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 226.186, 226.186, 226.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.660 - 2.210 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.660 | 2.300 |
| High resolution limit [Å] | 2.210 | 2.200 |
| Number of reflections | 43087 | |
| Completeness [%] | 86.3 | 69.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 298 | ADA, glycerol, ammonium sulfate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
