1R7A
Sucrose Phosphorylase from Bifidobacterium adolescentis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.040, 123.360, 151.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.770 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.19100 * |
Structure solution method | SIRAS |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.400 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.300 | 1.660 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.109 * | 0.530 * |
Number of reflections | 138899 | |
<I/σ(I)> | 4.3 | 0.9 |
Completeness [%] | 97.8 | 85.2 * |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 25 * | PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 25 * | PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 25 * | PEG 4000, sodium acetate, tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5-1.0 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.1 |
3 | 1 | reservoir | PEG4000 | 27 (%(w/v)) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | sodium acetate | 0.1 (M) |