1R6N
HPV11 E2 TAD complex crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 60.456, 60.456, 82.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.2095 |
| Rwork | 0.209 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HPV11 E2 TAb apo (1R6K) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 | 0.474 |
| Total number of observations | 70999 * | |
| Number of reflections | 11028 * | |
| <I/σ(I)> | 27.47 | 2.01 |
| Completeness [%] | 97.9 | 86.4 |
| Redundancy | 6.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 295 | Na Citrate, MPD, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 60 (mM) | ||
| 2 | 1 | drop | His-Lys-TAD | 10 (mg/ml) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.5 |
| 4 | 1 | reservoir | MPD | 35 (%) |
