1R6L
Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-28 |
Detector | MACSCIENCE |
Wavelength(s) | 0.9793, 0.9792, 0.9716 |
Spacegroup name | H 3 2 |
Unit cell lengths | 111.139, 111.139, 115.841 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.24500 * |
Structure solution method | MAD |
RMSD bond length | 0.005 * |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 * | 0.224 * |
Total number of observations | 415708 * | |
Number of reflections | 21859 | |
Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | Ammonium sulfate, CHES, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
6 | 1 | reservoir | 0.1 (M) | ||
7 | 1 | reservoir | CHES | 0.2 (M) | pH9.5 |