1R6K
HPV11 E2 TAD crystal structure
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790,0.9794,0.9743,0.9879 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 54.860, 169.900, 46.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.2566 |
| Rwork | 0.257 |
| R-free | 0.30300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.329 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.342 |
| Total number of observations | 68569 * | |
| Number of reflections | 7724 * | |
| <I/σ(I)> | 28.75 | 5.36 |
| Completeness [%] | 97.1 | 99 |
| Redundancy | 8.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | Na Succinate, PEG 5000, mme, Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium succinate | 0.1 (M) | pH5.0 |
| 2 | 1 | reservoir | PEG5000 MME | 18 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
