1R6C
High resolution structure of ClpN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.040, 51.990, 65.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| Rwork | 0.167 |
| R-free | 0.24326 |
| Structure solution method | MIR |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.026 | 0.052 |
| Number of reflections | 6243 | |
| <I/σ(I)> | 51.1 | 27.48 |
| Completeness [%] | 0.9 | 0.744 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 294 | 0.02M HEPES at pH 7.4 (protein solution) mixed 1:1 with 0.1M PIPES at pH 7.0, 35% (w/v) PEG 8000, 0.32M sodium citrate, HANGING DROP, temperature 294K |
