1R6B
High resolution crystal structure of ClpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 124.110, 124.110, 97.049 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.23504 |
Rwork | 0.234 |
R-free | 0.27784 |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 1.144 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.032 | 0.349 |
Number of reflections | 37627 | |
<I/σ(I)> | 21.9 | 1.36 |
Completeness [%] | 0.9 | 0.79 |
Redundancy | 2.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | hepes, potassium chloride, magnesium chloride, isopropanol, PEG 4000, glycerol, ADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |