1R5Q
Crystal Structure Analysis of Kai A from PCC7120
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2002-11-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 57.299, 57.299, 137.515 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.420 - 2.000 |
Rwork | 0.241 |
R-free | 0.26900 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 9566 | |
Completeness [%] | 98.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MgSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MgSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |